The effect of PDT on H. influenzae biofilm in vivo

Methods Sixteen bullae of 8 gerbils were injected with 200μl (107CFU/ml) of H. influenza and formation of biofilms in the bullae was obtained by 5 days. The bullae were divided into control, laser, photofrin, and PDT groups. The control group received no treatment. For laser group, 120 J/cm2 (100 mw x 20 min) of 632 nm LD laser was irradiated into the bullae by a fiber inserted directly into the bullae. For photofrin group, photofrin 40μl (1mg/ml) were injected into the bullae. For PDT group, photofrin same as in photofrin group was injected into the bullae and LD laser was irradiated into the bullae same way as in laser group. The mucosal tissues in bullae were examined by H/E staining, and SEM.